Balance
When the centrifuge is working, the rotor will rotate very fast, thus generating great centrifugal force, so it is crucial to properly balance the rotor during the rotation process, especially when the rotor is loaded with samples. Any imbalance of the rotor, including placing the centrifuge on an uneven or tilted workbench surface, will cause abnormal vibrations, which will damage the rotor. Ensure the longevity of your equipment with proper rotor balancing during high-speed rotation. Don’t risk damaged rotors due to improper setup, such as using an uneven or tilted workbench surface. Leave no room for abnormal vibrations with precise balance during every spin.
Since the centrifugal force generated during centrifugation is proportional to the sample mass and acceleration, accurate balancing becomes particularly important at high speeds. At high RCFs, even slight differences in sample mass can cause large imbalances in the rotor. To ensure accuracy and prevent imbalances, precise sample balancing is crucial, especially at high speeds where the centrifugal force is proportional to both sample mass and acceleration. Even the smallest differences in sample mass can create significant imbalances in the rotor at high RCFs.
RPM and RCF
RPM (revolutions per minute) and RCF (relative centrifugal force or g-force) are commonly used to describe centrifuge speed. RCF refers to the acceleration applied to the sample, for example 10,000 g means 10,000 times the force of gravity on the earth. RPM is not a very effective unit because the centrifugal force varies with the radius of the machine (the larger the radius, the greater the acceleration applied to the sample at the same speed). RCF is proportional to the radius of the rotor and the square of the RPM, so the speed setting needs to be converted to RCF to ensure that the correct centrifugal force is provided. RCF, or relative centrifugal force, is the preferred method for measuring centrifuge speed. It accurately reflects the acceleration applied to the sample by taking into account the radius of the machine and the square of the RPM. With RCF, you can ensure that the correct centrifugal force is provided for your samples, making it a more effective unit of measurement.
Centrifugation and Temperature
The centrifugal process generally generates heat because the movement of molecules and friction with the air causes the temperature inside the centrifuge to rise, which affects the stability of the sample. There are three main factors that affect the temperature inside the centrifuge: rotor material, rotor shape, and centrifugal speed. Experience the benefits of a perfectly balanced centrifuge. Our centrifuge is designed with materials, shapes, and speeds that minimize heat generation, ensuring the stability of your precious samples. Trust in our expert engineering for reliable and accurate results every time.
Rotor material: Materials such as steel and aluminum have high density and high thermal conductivity, which can effectively transfer heat and cool quickly. Materials such as polymers and carbon fiber are low-density materials (insulating materials) and help maintain a constant temperature. Expertly designed for maximum performance. The specially selected rotor materials, including high-density steel and aluminum and low-density polymers and carbon fiber, ensure efficient heat transfer and temperature regulation.
Rotor shape: Determines the airflow inside the centrifuge. Optimizing the airflow inside the centrifuge through rotor shape design is crucial to maintaining temperature. Improve your centrifuge efficiency with carefully designed rotor shape. The innovative design optimizes airflow, ensuring precise temperature control for accurate results every time.
Centrifugal speed: The centrifugal speed is proportional to the temperature rise. At higher speeds, more heat will be generated. Temperature control at your fingertips. The centrifugal speed is optimized for maximum heat generation, giving you precise control over the temperature rise.
Optimizing the airflow inside the centrifuge is essential for maintaining temperature, which is determined by the rotor shape design.
The degree of temperature rise depends largely on the maximum speed and shape of the rotor. It is important to understand the maximum speed of the centrifuge and the speed range that maintains the temperature range without changing the experimental results. It is essential to consider the maximum speed of the centrifuge and the corresponding temperature range to ensure the accurate results of your experiments. Knowing these factors will help you achieve the optimal temperature range necessary for your experiments.
Acceleration and deceleration
Many centrifuges offer the option to control the deceleration setting (brake) to stop faster and the effect on sample results.
Braking is particularly useful in centrifugation processes involving nucleic acid extraction or bacterial cell pelleting, as these processes are not affected by sudden stops. For experiments that are sensitive to sudden deceleration, such as isolation of peripheral blood mononuclear cells and gradient centrifugation, braking can cause remixing of the separation layers. Boost your experiments with the added assurance of consistent results. Braking is crucial in centrifugation procedures like nucleic acid extraction and bacterial cell pelleting, which are not impacted by unexpected hold-ups. Additionally, for sensitive tests like peripheral blood mononuclear cell isolation and gradient centrifugation, braking can prevent remixing between separation layers for more precise outcomes.
Therefore, centrifuges with different acceleration/deceleration settings provide users with the option to adjust the acceleration and deceleration speeds and more easily optimize experimental operations. Increase accuracy in experimental procedures with adjustable acceleration and deceleration speeds.
Centrifuge Particle Position
One of the applications of centrifuges is to separate bacterial cells, mammalian cells or nucleic acids into pellets. The angle of the rotor determines the position of the pellet. In the case of a horizontal swing rotor, the pellet is formed at the bottom of the centrifuge tube, and in the case of a fixed angle rotor, the pellet exists at a certain angle to the bottom of the centrifuge tube. Separate cells and molecules with ease and precision. The centrifuge’s swing or fixed angle rotor determines the position of the pellet, ensuring efficient separation every time.