Selection of centrifuge rotors
Material selection
The material selection of the rotor is mainly based on the speed of the centrifuge and the experimental requirements. The rotors of low-speed centrifuges (below 5000r/min) are generally made of ordinary steel, stainless steel and engineering plastics. The rotors of high-speed centrifuges (5000-50000r/min) and ultra-high-speed centrifuges (over 50000rpm) need to use high-strength and high-density materials, such as aluminum alloy or titanium alloy. Titanium alloy is an ideal choice for ultra-high-speed centrifuge rotors due to its excellent properties (such as low density, high specific strength and specific fracture toughness, good corrosion resistance, etc.), but the price is relatively high.
Type selection
The rotors of centrifuges are mainly divided into two categories: horizontal rotors and angle rotors. The choice of which rotor type depends mainly on the type of centrifugation (differential, speed band or isopycnic), speed and capacity range. Angle rotors have a certain angle between the centrifuge tube and the rotor axis (usually 14°-45°), which is suitable for separating particulate samples with obvious differences in sedimentation velocity, can quickly collect sediments, and can withstand high centrifugal forces. Horizontal rotors are suitable for centrifugal samples that require axisymmetric balance.
The choice of rotor type depends on the type of centrifugation, speed and capacity range. Angle rotors are designed specifically for separating samples with significantly different sedimentation velocities, can quickly collect sediments, and can withstand high centrifugal forces. In contrast, swing-out rotors are ideal for centrifugal samples that require axisymmetric balance.
Maintenance of centrifuge rotors
Installation and removal
The rotor should be installed and removed easily to avoid damage from falling. Remove the rotor vertically upwards and pay attention to protecting the drive shaft to prevent bumps and damage to the cone surface.
Cleaning and Maintenance
After centrifugation, the dirt and water on the inner and outer surfaces of the rotor should be cleaned in time to prevent corrosion and oxidation. It is recommended to use a neutral detergent and avoid using a brush with hard bristles or a brush with steel wire on the top to avoid damaging the rotor surface. After cleaning, the rotor should be inverted to dry and stored in a dry environment.
Balance and symmetrical placement:
Before centrifugation, ensure that the sample is balanced and the solutions in the corresponding centrifuge tubes should be of equal density and volume to maintain the axial symmetry of the sample. This helps reduce vibration and noise during centrifugation and improve the accuracy of experimental results.
Regular inspection and maintenance
Regularly check the rotor for corrosion spots, grooves, small cracks and other damages. If any, they should be promptly treated or replaced. At the same time, check whether the rubber seal is deformed or aged. If necessary, replace it in time to prevent external heat from penetrating.
Lubrication and sterilization
Regularly apply lubricating oil or vacuum silicone grease to the rotor thread surface and the part in contact with the rubber ring to reduce wear and extend service life. In addition, the rotor and most rotor accessories (except for specific materials) can be sterilized or disinfected under appropriate conditions to ensure the sterility of the experimental environment.
The selection and maintenance of centrifuge rotors involve many aspects, including materials, types, installation and removal, cleaning and maintenance, balance and symmetrical placement, and regular inspection and maintenance. Correct selection and effective maintenance of centrifuge rotors are essential to ensure the normal operation and extend the service life of the centrifuge.
Correct selection and proper maintenance of centrifuge rotors can give you peace of mind. From materials and installation to cleaning, inspection and balancing, every aspect is essential to ensure the reliable performance and long life of the centrifuge.