RPM :
The higher the rotational speed of the centrifuge, the greater the centrifugal force generated and the better the separation is usually. For example, when separating small particles or mixtures with small differences in density, a high speed can separate them more effectively. However, too high a speed may result in sample damage or overloading of the machine.
Centrifugation time :
If the centrifugation time is too short, the mixture may not be adequately separated, while too long a time may result in unnecessary energy consumption and over-processing of the sample. For example, when separating blood components, improper time control may affect the purity of the separation of plasma and blood cells.
Temperature :
Temperature has an effect on the separation. Some samples may undergo changes in their properties at high or low temperatures, which may affect the separation. For example, some biological samples may be inactivated at high temperatures.
Sample properties :
This includes the concentration, particle size, density difference, and viscosity of the sample. High sample concentration may lead to clogging, particle size and density difference is too small will increase the difficulty of separation, viscosity is not conducive to separation. For example, when separating viscous oils and impurities, it is relatively difficult to achieve the desired results.
Type of rotary head :
Different types of rotary heads, such as angular rotary heads and horizontal rotary heads, have different separation effects and application ranges. Angular heads are suitable for density gradient centrifugation, while horizontal heads are more suitable for separation of large-volume samples.
Centrifuge Balancing :
If the loaded samples are not evenly distributed within the centrifuge, this can cause the machine to become unbalanced, which not only affects the separation results, but can also damage the machine.
Machine maintenance and calibration :
Regular maintenance and accurate calibration ensure stable performance of the centrifuge and thus reliability of the separation results. Wear and tear of machine components or malfunctions that are not addressed in a timely manner can reduce the accuracy and efficiency of the separation.